plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111elSSN : 2287-8815
In Vitro Screening of Bioactivities of Kjellmaniella crassifolia Extracts
노윤이·윤현정·박선영·장미순1·이가정·박큰바위·하광수·손광태2·오재영2*
국립수산과학원 식품안전가공과, 1국립수산과학원 사료연구센터, 2국립수산과학원 서해수산연구소
The biofunctional properties of hot water extract (KHWE) and ethanol extract (KEE) of Kjellmaniella crassifolia were evaluated and their potential as natural functional ingredients was assessed. Antioxidant, anticancer, antimetastatic, anti-photoaging, anti-inflammatory, and antiadipogenic activities were investigated. KEE exhibited higher 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity, nitrite-scavenging activity, and total polyphenol content than KHWE. Both KHWE and KEE inhibited the proliferation of HT-1080, HT-29, and AGS cells and suppressed matrix metalloproteinase-9 expression, suggesting antimetastatic potential. In ultraviolet A-induced human dermal fibroblasts, both extracts reduced matrix metalloproteinase-1 expression, indicating anti-photoaging effects. In lipopolysaccharide- stimulated RAW 264.7 macrophages, both extracts inhibited intracellular reactive oxygen species and nitric oxide production, as well as inducible nitric oxide synthase and cyclooxygenase-2 expression, in a concentration- dependent manner. Furthermore, both extracts suppressed lipid accumulation and downregulated adipogenic transcription factors, including peroxisome proliferator-activated receptor γ, CCAAT/enhancer-binding protein α, and sterol regulatory element-binding protein 1c. These findings suggest that K. crassifolia extracts possess diverse biofunctional properties and may serve as promising natural ingredients for functional foods and nutraceutical applications.
Anticancer, Anti-inflammatory, Anti-obesity, Antioxidant, Kjellmaniella crassifolia