plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111 | elSSN : 2287-8815
plSSN : 0374-8111elSSN : 2287-8815
Preliminary Biological Activity Evaluation of Enzymatic Hydrolysatesfrom Fertilized Eggs of Olive Flounder Paralichthys olivaceus as aFunctional Bioactive Resource
김병훈·오수현1·신찬성1·허성표2*
제주국립대학교 기당해양과학원, 1㈜브이에스신비, 2제주국립대학교 해양생명과학과
This study investigated the potential industrial use of fertilized eggs of olive flounder Paralichthys olivaceus, an aquaculture by-product, as a functional bioactive ingredient. High-performance liquid chromatography confirmed that proteins isolated from fertilized eggs were identical when analyzed in two solvents [100% dimethyl sulfoxide and methanol:water (2:3, v/v)]. Enzymatic hydrolysates of egg protein showed no inhibitory effects on elastase or tyrosinase activity. However, ABTS+ radical scavenging assays revealed concentration-dependent antioxidant activity. In addition, evaluation of nitric oxide (NO) inhibition and cytotoxicity using the MTT assay demonstrated that the protein suppressed NO production in a dose-dependent manner, with negligible cytotoxicity. These findings highlight the antioxidant and anti?inflammatory potential of enzymatic hydrolysates derived from fertilized olive flounder eggs. As a sustainable fish by-product, these hydrolysates may serve as promising functional materials for industrial applications.
ABTS+ radical scavenging, By-product, HPLC, MTT assay, Olive flounder fertilized eggs